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International Journal of Toxicology
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Chromium Toxic Effect Monitoring Using Ozonation Method

T. Poznyak

Superior School of Chemical Engineering, National Polytechnic Institute of Mexico, Mexico D.F., Mexico

J. M. Puga

National School of Medicine and Homeopathic, National Polytechnic Institute of Mexico, Mexico D.F., Mexico

E. Kiseleva

Department of Polymer and Composite Materials, Institute of Chemical Physics of Russian Academy of Sciences, Moscow, Russia

L. Martinez

Department of Aquatic Toxicology, National School of Biological Science, National Polytechnic Institute of Mexico, Mexico D.F., Mexico

The hexavalent chromium toxicity (in vitro) to plasma, erythrocytes, and semen lipids was evaluated. The ozonation technique is suggested to realize the rapid measurements of the lipid peroxidation (LPO) by means of the double bond indexes (DB-index and DBcell-index) calculation. The obtained experimental results permit to conclude that it is possible to detect the chromium effect on LPO by ozonation. The DB-index and DBcell-index determination in the plasma, erythrocytes, and sperm can be considered as a measure of this effect Ozonation, suggested in this work, can provide the useful preliminary information for specialists and is a quantitative, fast, inexpensive, and simple method. In view of these comments, we conclude that the suggested ozonation method can be considered as the acceptable modern technique for the chromium toxic effect monitoring in vitro.

Key Words: Chromium Effect • Ozonation • Erythrocytes • Plasma • Semen

International Journal of Toxicology, Vol. 21, No. 3, 211-217 (2002)
DOI: 10.1080/10915810290096342
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