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Genotoxic Effects of Benzo[a]pyrene and Dibenzo[a,l]pyrene in a Human Lung Cell Line

Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina

Correspondence: Address correspondence to Dr. Carlos Golijow, CIGEBA, Facultad de Cs. Veterinarias, UNLP. Calle 60 y 118 S/N, B1900AVW La Plata, Argentina. E-mail:cgolijow{at}fcv.unlp.edu.ar

Several studies have shown that polycyclic aromatic hydrocarbons (PAHs) produce genotoxic effects in assays performed in vivo and in vitro. This study was undertaken to investigate the ability of benzo[a]pyrene (BP) and dibenzo[a,l]pyrene (DBP) to induce DNA damage in a human lung fibroblast cell line (MRC-5), using sister-chromatid exchanges test (SCEs), the comet assay, and evaluating point mutations in codon 12 of the K-ras protooncogene by polymerase chain reaction–single-strand conformation polymorphisms (PCR-SSCPs) and restriction fragment length polymorphisms (RFLP)-enriched PCR methods. Sister-chromatid exchanges frequencies were significantly increased in cells exposed to benzo[a]pyrene and dibenzo[a,l]pyrene in relation to controls (p < .001). Using the standard alkaline comet assay, significant differences between groups were found for the variable comet moment (CM) when cells were exposed to BP (p < .001) and DBP (p < .001). Nevertheless, PCR-SSCP and RFLP-enriched PCR methods did not show any association between treatments with BP and DBP and K-ras point mutations. The data presented in this study indicated that BP and DBP induced both DNA strand breaks and sister-chromatid exchanges but not significant point mutations at codon 12 of K-ras gene in the MRC-5 cell line.

Key Words: Benzo[a]pyrene • Comet Assay • Dibenzo[a, l]pyrene • Genotoxicity • K-ras Protooncogene

International Journal of Toxicology, Vol. 25, No. 1, 49-55 (2006)
DOI: 10.1080/10915810500488411


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