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Protective Role of Melatonin and Coenzyme Q10 in Ochratoxin A Toxicity in Rat Liver and Kidney

Department of Biochemistry, Medical School, Osmangazi University, Eskiehir, Turkey

Erinc Aral

Department of Histology, Medical School, Osmangazi University, Eskiehir, Turkey

Filiz Ozdemir

Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskiehir, Turkey

Sema Uslu
Ozkan Alatas
Omer Colak

Department of Biochemistry, Medical School, Osmangazi University, Eskiehir, Turkey

Correspondence: Address correspondence to Dr. Filiz Ozdemir, Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskiehir, Turkey. E-mail:fozdemir3{at}anadolu.edu.tr

Melatonin (MEL) and coenzyme Q₁₀ (CoQ₁₀) both display antioxidant and free radical scavenger properties. In the present study, the effect of MEL and CoQ₁₀ on the oxidative stress and fibrosis induced by ochratoxin A (OTA) administration in rats was investigated. Rats were divided into five equal groups, each consisting of seven rats: (1) controls; (2) OTA-treated rats (289 µg/kg/day); (3) OTA+MEL–treated rats (289 µg/kg/day OTA + 10 mg/kg/day MEL); and (4) OTA+CoQ₁₀–treated rats (289 µg/kg/day OTA +1 mg/100 g/day body weight (bw) CoQ₁₀). After 4 weeks of treatment, the level of malondialdehyde (MDA), glutathione peroxidase (GPx), and hydroxyproline (Hyp) were measured in the homogenates of liver and kidney. In the OTA-treated group, the levels of MDA and Hyp in both liver and kidney were significantly increased when compared with the levels of control, whereas GPx activities decreased. In OTA+MEL–treated rats, the levels of MDA and Hyp in both liver and kidney were significantly decreased when compared with the levels of OTA-treated rats; however; GPX activities increased. In the OTA+CoQ10–treated group, the levels of MDA and Hyp were decreased when compared with the levels of OTA-treated rats, whereas GPx activities increased. In the OTA+CoQ ₁₀–treated group, the levels of MDA, Hyp, and GPx were not significantly changed in kidney when compared with OTA-treated group. MEL has a protective effect against OTA toxicity through an inhibition of the oxidative damage and fibrosis both liver and kidney. Although CoQ₁₀ has protective effect against OTA toxicity in liver tissue, it has no effect in kidney tissue.

Key Words: Coenzyme Q₁₀ • Free Radicals • Hydroxyproline • Melatonin • Ochratoxin A

International Journal of Toxicology, Vol. 26, No. 1, 81-87 (2007)
DOI: 10.1080/10915810601122893


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