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International Journal of Toxicology
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Articles

Gene Expression Analysis on the Early Development of Pig Embryos Exposed to Malathion

Zayil Salazar

Doctorado en Biología Experimental, CBS, Universidad Autónoma Metropolitana, Iztapalapa, México, D. F. México

Yvonne Ducolomb
Miguel Betancourt
Edmundo Bonilla

Laboratorio de Análisis de la Expresión Génica, Departamento Ciencias de la Salud, Universidad Autónoma Metropolitana, Iztapalapa, México, D. F. México

Leticia Cortés
Fidel Hernández-Hernández

Laboratorio de Patología, CINVESTAV., México, D. F. México

Humberto González-Márquez

Laboratorio de Análisis de la Expresíon Génica, Departamento Ciencias de la Salud, Universidad Autónoma Metropolitana, Iztapalapa, México, D. F. México

Correspondence: Address correspondence to Dr. Humberto González-Márquez, Universidad Autónoma Metropolitana, Departamento Ciencias de la Salud, San Rafael Atlixco 186, Iztapalapa, México D. F. C. P. 09340, México. E-mail:hgm{at}xanum.uam.mx

Malathion is a widely used pesticide and there is evidence that it could alter mammal’s germ and somatic cells, as well as cell lines. There are not enough studies showing how the nonacute malathion doses affect gene expression. This study analyzes gene expression alterations in pig morular embryos exposed in vitro, for 96 h, to several malathion concentrations after in vitro fertilization. cDNA libraries of isolated morular embryos were created and differential screenings performed to identify target genes. Seven clones were certainly identified. Genes related to mitochondrial metabolism as cytochrome c subunits I and III, nuclear genes such as major histocompatibility complex I (MHC I), and a hypothetical protein related with a splicing factor were the target of malathion’s deregulation effect. The widespread use of malathion as a pesticide should be regarded with reproductive implications and more detailed analysis would yield more about molecular mechanisms of malathion injury on embryo cells.

Key Words: Gene Expression • In Vitro Fertilization • Malathion • Pig Embryo Culture

International Journal of Toxicology, Vol. 26, No. 2, 143-149 (2007)
DOI: 10.1080/10915810701226263
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