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Combined Effects of Vitamin C, Vitamin E, and Sodium Selenate Supplementation on Absolute Ethanol-Induced Injury in Various Organs of Rats

Department of Chemistry, Faculty of Engineering, Istanbul University, Istanbul, Turkey

Sadakat Ozdil

Department of Internal Medicine, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey

Sehnaz Bolkent

Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey

Correspondence: Address correpondence to Prof. Dr. Refiye Yanardag, Istanbul University, Faculty of Engineering, Department of Chemistry 34320 Avcilar, Istanbul, Turkey. E-mail:refiyeyanardag{at}yahoo.com;yanardag{at}istanbul.edu.tr

In this study, the effect of combination of vitamin C (ascorbic acid), vitamin E (-tocopherol), and selenium (sodium selenate) on ethanol-induced liver and intestine injury in rats was investigated. The ethanol-induced injury was produced by the administration of 1 ml of absolute ethanol to each rats. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg), and sodium selenate (Se) (0.5 mg/kg) for 3 days; 1 h after the final antioxidant administration, they were sacrificed. Lipid peroxidation and glutathione levels, catalase (CAT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GP_x) activities were determined in liver and intestine tissues. Myeloperoxidase (MPO), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), -glutamyltransferase (GGT) were determined in liver tissue. Also, CAT activity, urea, creatinine, uric acid, and total lipid levels were determined in serum samples. In the ethanol group, serum urea, creatinine, uric acid, and total lipid levels; liver and intestine LDH; liver MPO, AST, ALP, ALT, and GGT activities; and liver and intestine LPO levels increased, whereas serum CAT activity, liver and intestine GSH levels, and CAT, SOD, and GP_x activities decreased. On the other hand, treatment with vitamin C, vitamin E, and Se reversed these effects. As a result of these findings, we can say that the combination of vitamin C, vitamin E, and selenium has a protective effect on ethanol-induced changes in lipid peroxidation, glutathione levels, and antioxidant enzyme activities in liver and intestine tissues, and in some serum parameters of rats.

Key Words: ANOVA, analysis of variance • ROS, reactive oxygen species • LPO, lipid peroxidation • MDA, malondialdehyde • GSH, glutathione • CAT, catalase activity • TBARS thiobarbituric acid-reactive substances • LDL, low density lipoprotein • NADH, nicotinamide adenine dinucleotide, reduced form • (LDH) lactate dehydrogenase • (SOD) superoxid dismutase • (GP_x) glutathione peroxidase • (MPO) Myeloperoxidase • (AST) aspartate transaminase • (ALT) alanine transaminase • (ALP) alkaline phosphatase • (GGT) gamma glutamyl transferase

International Journal of Toxicology, Vol. 26, No. 6, 513-523 (2007)
DOI: 10.1080/10915810701707296


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